Description
Microscope head
Trinocular head with infinity-corrected optics. Eyepiece tubes can rotate 360°. The user can adjust eye relief on the eyepiece to fit their height. The digital camera is mounted in the trinocular tube.
Revolving nosepiece
Five objectives. A free slot is used for centering the reflected light source. An additional objective can also be installed in the free slot in order to achieve extra magnification.
The revolving nosepiece with objectives is oriented toward the interior – the user can see the objective inserted into the optical path, and the space above the stage is free.
Objectives
Infinity plan achromatic objectives and infinity plan fluorite objectives. Standard configuration includes 4x, 10x, and 100x objectives for brightfield microscopy and a 40x objective for fluorescence microscopy.
Focusing mechanism
Coaxial coarse and fine focusing knobs are located at the base of the microscope on both sides. The user can place their hands on the table and take a relaxed pose while observing. The focusing adjustment is smooth and effortless. On the right, there is a coarse focusing lock knob for quick adjustments after changing the specimen. On the left, there is a coarse tension adjustment ring for further adjustment.
Stage
The stage does not have an X-axis positioning rack, which ensures its ergonomic operation. The mechanical stage with a belt-driven mechanism allows for the smooth movement of the object. The slide holder is fixed with two screws and can be removed for easy operation. For reflected light observations, a black stage plate should be placed on the stage to remove stray light.
Reflected light illuminator
The fluorescence excitation source is a 100W mercury lamp. It is extremely bright and has a wide spectrum of wavelengths. The mercury lamp has discrete peaks, allowing you to work with many fluorochromes. The reflected light illuminator contains four excitation filters: ultraviolet (UV), violet (V), blue (B), and green (G). The mercury lamp is located in the lamphouse and can be centered in three planes. The heat radiation is conducted away from the lamphouse so that the lamp does not overheat. There is a mount that allows for safe and quick lamp replacement.
Köhler illumination in reflected and transmitted light
Setting up the Köhler illumination enhances the image quality of a specimen. With such illumination, you can achieve maximum resolution on each objective and uniform illumination of the field of view with no darkening at the edges. The object of study is in sharp focus, and the image artifacts are removed.
Condenser
The condenser has a slot for a darkfield slider or a phase contrast slider. The usage of slider saves time when switching between microscopy techniques in transmitted light.
Transmitted light source
The transmitted light illuminator has a 30W halogen bulb. Halogen bulbs emit light with a color temperature that allows for comfortable work. The 30W bulb is bright enough to observe using 4x to 100x magnification objectives.
Accessories
There is a line of accessories designed for the microscope. Optional objectives provide additional magnification. The optional 10x/0.35 fluorite objective is used for capturing an image of a specimen with a resolution of 0.5μm over a large field of view. Eyepieces that extend the magnification range of the microscope. Optional eyepieces help you maximize the potential of the objective you use most often. A phase contrast device, a darkfield condenser, and a polarization device offer more microscopy techniques so that you can study the specimens that are invisible in brightfield or fluorescent light. A digital camera that outputs the microscope image on a monitor and stores files along with software that takes real-time measurements of specimens. A calibration slide for measuring objects that can be combined with an eyepiece with a scale or the camera software.
Specifications
Microscope design: upright
Image field: flat-field
Reflected light microscopy techniques: fluorescence
Transmitted light microscopy techniques: brightfield, darkfield (optional), polarized light (optional), phase contrast (optional)
Microscope magnification, x: 40–1000 basic configuration (*optional: 40–1250/1500/2000/2500)
Tube length: infinity (∞)
Microscope head type: trinocular
Microscope head: Gemel head (Siedentopf, 360° rotation)
Inclination: 30°
Microscope head magnification, x: 1
Interpupillary distance, mm: 48–75
Eyepiece diameter, mm: 30
Eyepieces, x/field, mm: 10х/22mm, eye relief: 10mm (*optional: 10x/22mm with a scale, 12.5x/14; 15x/15; 20x/12; 25x/9)
Revolving nosepiece: 5 objectives
Optical design: infinity plan achromatic objectives and infinity plan achromatic objectives, fluo; parfocal distance: 45mm
Objectives, x/aperture/working distance, mm: PL 4x/0.10/19.8; PL 10x/0.25/5.0; PL FL 40x/0.85/0.42; PL 100x/1.25/0.36 (oil) (*optional: PL FL 10x/0.35/2.37; PL 20х/0.40/8.8; PL 60x/0.80 ∞/0.17 WD 0.46mm)
Spring-loaded retractable assembly: 40, 60х, 100x (oil)
Stage: double layer mechanical stage, stage size: 180mm×150mm, without a positioning rack
Moving range, mm: 75/50
Condenser: Abbe condenser, N.A. 1.25, center-adjustable, height-adjustable, adjustable diaphragm, a slot for a darkfield slider and phase contrast slider, dovetail mount
Field diaphragm: adjustable iris diaphragm
Focusing mechanism: coaxial coarse and fine focusing knobs located on both sides
Coarse focusing travel, mm: 21
Coarse focusing travel, mm/circle: 39.8
Fine focusing scale value, μm: 2
Coarse focusing tension adjusting knob: +
Coarse focusing lock knob: +
Illumination: transmitted and reflected light
Reflected light source: Mercury lamp 100W
Transmitted light source: 12V, 30W halogen bulb, brightness-adjustable
Fluorescence filter: filter type, excitation wavelength/dichroic mirror/emission wavelength: ultraviolet (UV), 320–380nm/425 nm/435 nm; violet (V), 380–415nm/455nm/475nm; blue (B), 450–490nm/505nm/515nm; green (G), 495–555nm/585nm/595nm
Phase contrast device: *optional: phase contrast turret condenser, phase contrast objective (10x, 20x, 40x, 100x), centering telescope
Darkfield condenser: *optional: dry darkfield condenser (for use with a dry objective); oil darkfield condenser (for use with a 100x immersion objective lens); darkfield slider
Polarization device: *optional: polarizer placed on the collector; analyzer inserted in the slot above the revolving nosepiece
Power supply, V/Hz: AC power supply, 85–265/50/60
Operating temperature range, °C (°F): 5… 35 (41… 95)
Operating humidity range, %: 20… 80