The darkfield slider is used for the darkfield microscopy technique. The darkfield microscopy is used for studying unstained transparent samples. Such samples absorb light weakly and, therefore, they are indistinguishable when observed using the brightfield microscopy technique.
The slider is a plate with two round holes. One slot is free for brightfield microscopy. The second slot has a darkfield diaphragm built into it. The slider is installed in the Abbe condenser slot and is used instead of a dry darkfield condenser for objectives with apertures up to 0.9. The slider makes it easy to switch between different microscopy techniques.
To get a contrast image of the sample using the darkfield microscopy technique, it is recommended to use a cover glass that is 0.17mm thick and a cover slip that is 1.2mm thick.