MAGUS Lum D400

83016
4999,95
MAGUS Lum D400 Fluorescence Digital Microscope
With a camera. Magnification: 40–1000x.Trinocular head, plan achromatic, and plan fluorite objectives, reflected light source – 100W mercury lamp, transmitted light source – 30W halogen lamp, Köhler illumination in reflected and transmitted light

The microscope is designed for studying specimens using the fluorescence microscopy technique in reflected light and brightfield technique in transmitted light. The additional equipment allows for darkfield, polarization, and phase contrast microscopy. Fluorescence microscopy is based on the ability of some substances to glow when exposed to the light of a certain part of the spectrum. The specimens are exposed to invisible short-wave ultraviolet light or violet, blue, and green light. The wavelength of the emitted light will be longer than the wavelength of the excitation light. The specimen glows blue, cyan, green-yellow, or red light, respectively. Some specimens glow by themselves while others glow after treatment with fluorochromes. The fluorescent microscope is used to study pathogens of tuberculosis, chlamydia, rabies, herpes, and other diseases. The microscope can also be used for DNA and chromosome analysis, bone marrow and blood smears, forensic and pharmacological examination, veterinary control studies, and sanitary and epidemiological inspection.
Description
Key features
Package
Microscope specifications
Camera specifications
References and downloads

Description

Digital camera
The MAGUS CLM10 digital camera is designed for use in fluorescence and darkfield microscopy techniques.
The camera is equipped with a 2.3MP sensor and produces realistic images at a resolution of 1920х1200px. It is recommended for 40x, 60x, and 100x objectives. When observing with medium magnification objectives, the camera will show finer details.
Video is recorded at 120fps at maximum resolution. Videos are smooth, with soft and subtle transitions between frames. The movement of the object is displayed in real time with no delays. The camera makes it easy to work with objects that are moving and is suitable for classroom demonstrations.
The camera is equipped with a USB3.0 interface. The data transfer speed is 10 times faster than USB2.0 cameras. The high-speed camera is recommended for professional laboratories, research, or university training.

Microscope head
Trinocular head with infinity-corrected optics. Eyepiece tubes can rotate 360°. The user can adjust eye relief on the eyepiece to fit their height. The digital camera is mounted in the trinocular tube.

Revolving nosepiece
Five objectives. A free slot is used for centering the reflected light source. An additional objective can also be installed in the free slot in order to achieve extra magnification.
The revolving nosepiece with objectives is oriented toward the interior – the user can see the objective inserted into the optical path, and the space above the stage is free.

Objectives
Infinity plan achromatic objectives and infinity plan fluorite objectives. Standard configuration includes 4x, 10x, and 100x objectives for brightfield microscopy and a 40x objective for fluorescence microscopy.

Focusing mechanism
Coaxial coarse and fine focusing knobs are located at the base of the microscope on both sides. The user can place their hands on the table and take a relaxed pose while observing. The focusing adjustment is smooth and effortless. On the right, there is a coarse focusing lock knob for quick adjustments after changing the specimen. On the left, there is a coarse tension adjustment ring for further adjustment.

Stage
The stage does not have an X-axis positioning rack, which ensures its ergonomic operation. The mechanical stage with a belt-driven mechanism allows for the smooth movement of the object. The slide holder is fixed with two screws and can be removed for easy operation. For reflected light observations, a black stage plate should be placed on the stage to remove stray light.

Reflected light illuminator
The fluorescence excitation source is a 100W mercury lamp. It is extremely bright and has a wide spectrum of wavelengths. The mercury lamp has discrete peaks, allowing you to work with many fluorochromes. The reflected light illuminator contains four excitation filters: ultraviolet (UV), violet (V), blue (B), and green (G). The mercury lamp is located in the lamphouse and can be centered in three planes. The heat radiation is conducted away from the lamphouse so that the lamp does not overheat. There is a mount that allows for safe and quick lamp replacement.

Köhler illumination in reflected and transmitted light
Setting up the Köhler illumination enhances the image quality of a specimen. With such illumination, you can achieve maximum resolution on each objective and uniform illumination of the field of view with no darkening at the edges. The object of study is in sharp focus, and the image artifacts are removed.

Condenser
The condenser has a slot for a darkfield slider or a phase contrast slider. The usage of slider saves time when switching between microscopy techniques in transmitted light.

Transmitted light source
The transmitted light illuminator has a 30W halogen bulb. Halogen bulbs emit light with a color temperature that allows for comfortable work. The 30W bulb is bright enough to observe using 4x to 100x magnification objectives.

Accessories
There is a line of accessories designed for the microscope. Optional objectives provide additional magnification. The optional 10x/0.35 fluorite objective is used for capturing an image of a specimen with a resolution of 0.5μm over a large field of view. Eyepieces that extend the magnification range of the microscope. Optional eyepieces help you maximize the potential of the objective you use most often. A phase contrast device, a darkfield condenser, and a polarization device offer more microscopy techniques so that you can study the specimens that are invisible in brightfield or fluorescent light. A digital camera that outputs the microscope image on a monitor and stores files along with software that takes real-time measurements of specimens. A calibration slide for measuring objects that can be combined with an eyepiece with a scale or the camera software.

Key features

Key features of the microscope:
  • Study of specimens emitting fluorescence when exposed to excitation light
  • Microscopy techniques: fluorescence in reflected light, brightfield in transmitted light
  • Trinocular head with adjustable eye relief and a vertical tube for mounting a digital camera
  • Reflected light illuminator: 100W mercury lamp of a wide wavelength spectrum
  • Fluorescence filters: ultraviolet (UV), violet (V), blue (B), green (G)
  • Transmitted light illuminator: 30W halogen bulb providing bright natural light
  • Köhler illumination in reflected and transmitted light
  • Simple stage without a positioning rack
  • Wide range of compatible optional accessories

Key features of the camera:
  • For fluorescence and darkfield microscopy with 40x, 60x, and 100x objectives
  • 2.3MP resolution for getting detailed images at high magnification; low noise level, low-power dissipation
  • 120fps for observing moving objects, recording video, and moving the sample without jerkiness or delays
  • Global shutter for fast signal readout from the sensor, increased image brightness, and no false optical effects when observing moving objects
  • SONY Exmor monochrome CMOS backlit sensor provides low noise level and high light sensitivity even in low-light conditions. You will get clearer, brighter, and more color-saturated images.
  • USB3.0 interface for fast, smooth data transfer
  • Software with photo, video recording, editing, external display functions, linear and angular measurements

Package

  • MAGUS CLM10 Digital Camera (digital camera, USB cable, installation CD with drivers and software, user manual and warranty card)
  • Base with a power input, transmitted light source and condenser, focusing mechanism, stage, and revolving nosepiece
  • Reflected light illuminator
  • Mercury lamphouse
  • Trinocular head
  • Infinity plan achromatic objective: PL 4x/0.10 WD 19.8mm
  • Infinity plan achromatic objective: PL 10x/0.25 WD 5.0mm
  • Infinity plan achromatic objective, fluo: PL FL 40x/0.85 (spring-loaded) WD 0.42mm
  • Infinity plan achromatic objective, fluo: PL 100x/1.25 (spring-loaded, oil) WD 0.36mm
  • Eyepiece 10x/22mm with long eye relief (2 pcs.)
  • UV shield
  • C-mount adapter 1x
  • Hex key wrench
  • Mercury lamphouse power supply
  • Power cord
  • Reflected light illuminator power cord
  • Dust cover
  • User manual and warranty card

Available on request:
  • 10x/22mm eyepiece with a scale
  • 12.5x/14mm eyepiece (2 pcs.)
  • 15x/15mm eyepiece (2 pcs.)
  • 20x/12mm eyepiece (2 pcs.)
  • 25x/9mm eyepiece (2 pcs.)
  • Infinity plan achromatic objective, fluo: PL FL 10x/0.35WD 2.37mm
  • Infinity plan achromatic objective: PL 60x/0.80 ∞/0.17 WD 0.46mm
  • Phase contrast device
  • Darkfield condenser
  • Immersion darkfield condenser
  • Darkfield slider
  • Polarization device
  • Calibration slide

Microscope specifications

Microscope design: upright

Image field: flat-field

Reflected light microscopy techniques: fluorescence

Transmitted light microscopy techniques: brightfield, darkfield (optional), polarized light (optional), phase contrast (optional)

Microscope magnification, x: 40–1000 basic configuration (*optional: 40–1250/1500/2000/2500)

Tube length: infinity (∞)

Microscope head type: trinocular

Microscope head: Gemel head (Siedentopf, 360° rotation)

Inclination: 30°

Microscope head magnification, x: 1

Interpupillary distance, mm: 48–75

Eyepiece diameter, mm: 30

Eyepieces, x/field, mm: 10х/22mm, eye relief: 10mm (*optional: 10x/22mm with a scale, 12.5x/14; 15x/15; 20x/12; 25x/9)

Revolving nosepiece: 5 objectives

Optical design: infinity plan achromatic objectives and infinity plan achromatic objectives, fluo; parfocal distance: 45mm

Objectives, x/aperture/working distance, mm: PL 4x/0.10/19.8; PL 10x/0.25/5.0; PL FL 40x/0.85/0.42; PL 100x/1.25/0.36 (oil) (*optional: PL FL 10x/0.35/2.37; PL 60x/0.80 ∞/0.17 WD 0.46mm)

Spring-loaded retractable assembly: 40, 60х, 100x (oil)

Stage: double layer mechanical stage, stage size: 180mm×150mm, without a positioning rack

Moving range, mm: 75/50

Condenser: Abbe condenser, N.A. 1.25, center-adjustable, height-adjustable, adjustable diaphragm, a slot for a darkfield slider and phase contrast slider, dovetail mount

Field diaphragm: adjustable iris diaphragm

Focusing mechanism: coaxial coarse and fine focusing knobs located on both sides

Coarse focusing travel, mm: 21

Coarse focusing travel, mm/circle: 39.8

Fine focusing scale value, μm: 2

Coarse focusing tension adjusting knob: +

Coarse focusing lock knob: +

Illumination: transmitted and reflected light

Reflected light source: Mercury lamp 100W

Transmitted light source: 12V, 30W halogen bulb, brightness-adjustable

Fluorescence filter: filter type, excitation wavelength/dichroic mirror/emission wavelength: ultraviolet (UV), 320–380nm/425 nm/435 nm; violet (V), 380–415nm/455nm/475nm; blue (B), 450–490nm/505nm/515nm; green (G), 495–555nm/585nm/595nm

Phase contrast device: *optional: phase contrast turret condenser, phase contrast objective (10x, 20x, 40x, 100x), centering telescope

Darkfield condenser: *optional: dry darkfield condenser (for use with a dry objective); oil darkfield condenser (for use with a 100x immersion objective lens); darkfield slider

Polarization device: *optional: polarizer placed on the collector; analyzer inserted in the slot above the revolving nosepiece

Power supply, V/Hz: AC power supply, 85–265/50/60

Operating temperature range, °C (°F): 5… 35 (41… 95)

Operating humidity range, %: 20… 80

Camera specifications

Sensor: SONY Exmor CMOS

Color/monochrome: monochrome

Megapixels: 2.3

Maximum resolution, pix: 1920х1200

Sensor size: 1/1.2" (11.25x7.03)

Pixel size, μm: 5.86x5.86

Light sensitivity: 1016mV with 1/30s

Signal/noise ratio: 0.15mV with 1/30s

Exposure: 0.244ms–2s

Video recording: +

Frame rate, fps at resolution: 120@1920x1200; ADC digit capacity (bit); 8/12 (selectable)

Place of installation: trinocular tube, eyepieces tube instead of an eyepiece

Image format: *.jpg, *.bmp, *.png, *.tif

Video format: recording: *.wmv, *.avi, *.h264 (Win 8 or newer), *h265 (Win 10 or newer)

Spectral range, nm: 380–650 (with IR filter and anti-reflective filter)

Shutter type: Global shutter

White balance: auto/manual

Exposure control: auto/manual

Software features: image size, brightness, exposure time

Output: USB3.0, 5Gb/s

System requirements: Windows 8/10/11 (32 and 64 bit), Mac OS X, Linux, up to 2.8GHz Intel Core 2 or higher, minimum 2GB RAM, USB3.0 port, CD-ROM, 17" or larger display

Software: MAGUS View

Mount type: C-mount

Body: CNC aluminum alloy

Power supply: DC current, 5V, with the USB port of the PC

Operating temperature range, °C (°F): –10... 50 (14... 122)

Operating humidity range, %: 30… 80