MAGUS Epi-FL06 Epi-Fluorescence Attachment
85157
MAGUS Epi-FL06 Epi-Fluorescence Attachment, mercury lamp, fluorescence filter cubes В/G/UV/V/R/FITC/TRITC/DAPI
For fluorescence observations
The epi-fluorescence attachment is used to employ the fluorescence technique.
The fluorescence technique is based on the ability of substances to emit light when excited by the light of a certain wavelength. Some specimens exhibit autofluorescence, while others should be treated with fluorochromes.
For fluorescence observations
The epi-fluorescence attachment is used to employ the fluorescence technique.
The fluorescence technique is based on the ability of substances to emit light when excited by the light of a certain wavelength. Some specimens exhibit autofluorescence, while others should be treated with fluorochromes.
The attachment is mounted into the optical path between the microscope head and stand. There is a 6-position rotating turret inside the attachment with fluorescence filters. Five fluorescence filter cubes are installed in the turret at the same time (user-selectable). The required filter is introduced into the optical path by rotating the ring. One position of the turret corresponds to the brightfield observations. The side knobs are used to adjust the opening of the field and aperture diaphragms. A set of fluorescence filter cubes includes filters of different fluorescence wavelengths: broadband blue (B), green (G), ultraviolet (UV), violet (V), narrow-band red (R), and narrow-band filters for DAPI, FITC, and TRITC fluorochromes.
The fluorescence excitation source is a mercury lamp. It features high brightness and a wide spectrum of wavelengths. The lamp spectrum is peaked, which makes it possible to work with a variety of fluorescent dyes. The mercury lamp is located in the lamphouse. The lamphouse dissipates heat, and the lamp does not overheat.
To employ the fluorescence technique, use the MAGUS Epi-FL06 attachment together with the MAGUS PlanF S-Apo fluorescent objective set. The plan semi-apochromatic fluorescent objective enhances the microscope’s resolving power and reduces chromatic and spherical aberrations. The image in the eyepieces and through the digital camera is cleaner, has more contrast, and it is brighter than with a regular plan achromatic objective.
The high numerical aperture of fluorescent objectives enhances the microscope’s resolving power, but at the same time, it reduces the depth of field. Therefore, for transmitted light observations, we recommend using regular objectives from the microscope set with a greater depth of field.
The fluorescence excitation source is a mercury lamp. It features high brightness and a wide spectrum of wavelengths. The lamp spectrum is peaked, which makes it possible to work with a variety of fluorescent dyes. The mercury lamp is located in the lamphouse. The lamphouse dissipates heat, and the lamp does not overheat.
To employ the fluorescence technique, use the MAGUS Epi-FL06 attachment together with the MAGUS PlanF S-Apo fluorescent objective set. The plan semi-apochromatic fluorescent objective enhances the microscope’s resolving power and reduces chromatic and spherical aberrations. The image in the eyepieces and through the digital camera is cleaner, has more contrast, and it is brighter than with a regular plan achromatic objective.
The high numerical aperture of fluorescent objectives enhances the microscope’s resolving power, but at the same time, it reduces the depth of field. Therefore, for transmitted light observations, we recommend using regular objectives from the microscope set with a greater depth of field.
- MAGUS Bio 290T Biological Microscope
- Epi-fluorescence attachment
- В/G/UV/V/R/FITC/TRITC/DAPI fluorescence filter cubes
- Mercury lamphouse with a power supply
- UV shield
Excitation light source: mercury lamp
Fluorescence filters: filter type, excitation wavelength / dichroic mirror / emission wavelength: blue (B), 460–495nm / 505nm / 510nm; green (G), 510–550nm / 570nm / 575nm; ultraviolet (UV), 330–385nm / 410nm / 420nm; violet (V), 400–410nm / 455nm / 460nm; red (R), 620–650nm / 660nm / 670–750nm
Fluorescence filters: fluorochrome, excitation wavelength / dichroic mirror / emission wavelength: DAPI, 360–390nm / 415nm / 435–485nm; FITC, 460–495nm / 505nm / 510–550nm; TRITC, 528–553nm / 565nm / 578–633nm
Fluorescence filters: filter type, excitation wavelength / dichroic mirror / emission wavelength: blue (B), 460–495nm / 505nm / 510nm; green (G), 510–550nm / 570nm / 575nm; ultraviolet (UV), 330–385nm / 410nm / 420nm; violet (V), 400–410nm / 455nm / 460nm; red (R), 620–650nm / 660nm / 670–750nm
Fluorescence filters: fluorochrome, excitation wavelength / dichroic mirror / emission wavelength: DAPI, 360–390nm / 415nm / 435–485nm; FITC, 460–495nm / 505nm / 510–550nm; TRITC, 528–553nm / 565nm / 578–633nm
