MAGUS Lum VD500L

83022
10999,95
MAGUS Lum VD500L Fluorescence Inverted Digital Microscope
With a camera. Magnification: 100–400x. Trinocular head, plan achromatic objectives, 5W LED transmitted light and reflected light illumination, Köhler illumination

The microscope is intended for studying specimens using the fluorescence microscopy technique in reflected light and using the brightfield and phase contrast techniques in transmitted light.
The fluorescence microscopy is based on the ability of some substances to glow when exposed to light of a certain part of the spectrum. The illuminator contains three filters that provide optimal conditions for fluorescence excitation of the commonly used fluorochromes DAPI, FITC, and TRITC.
The microscope is used for the examination of liquid precipitates, cell colonies, living cells, tissue cultures, and other samples in nutrient medium in glassware up to 55mm with the bottom thickness of 1.2mm. The microscope uses special objectives for working with such glassware. The illuminator stand is tiltable for use with Petri dishes or flasks up to 165mm. The microscope is suitable for immunochemical diagnostics and chromosome analysis as well as the detection of latent bacterial and viral infections.
Description
Key features
Package
Microscope specifications
Camera specifications
References and downloads

Description

Digital camera
The MAGUS CLM90 digital camera is designed for use in fluorescence and darkfield microscopy techniques.
The camera is equipped with a 7.1MP sensor and produces realistic images at a resolution of 3200x2200px. The camera is recommended for use with 4x, 10x, 20x, and 40x objectives. When working with low magnification objectives, the camera will allow you to see the finer details.
Video is recorded at 51.3fps or 133.8fps depending on its resolution. Videos are smooth with soft and subtle transitions between frames. The movement of the sample is displayed in real time with no delays. The camera makes it easy to work with objects that are moving and is suitable for classroom demonstrations.
The camera is equipped with a USB3.0 interface. The data transfer speed is 10 times faster than USB2.0 cameras. The high-speed camera is recommended for professional laboratories, research, or university training.

Microscope head
Tube length – infinity (∞). When assembling the microscope, the user can rotate the eyepiece tubes 180° and adjust eye relief to fit their height. There is a trinocular microscope head.
The monitor is mounted in the vertical tube of the trinocular head and the digital camera – in the camera side port. The trinocular head has an 80:20 beam splitter and there is a 2-position beam splitter on the body (100:0/0:100).

Revolving nosepiece
The 6-objectives revolving nosepiece is mounted on a stand under the stage.

Objectives
Infinity plan achromatic objectives with a long working distance for dishes with a bottom thickness of 1.2mm.
Three objectives are intended for brightfield and fluorescence microscopy techniques, and the other 3 objectives are for the phase contrast technique.

Focusing mechanism
Coaxial coarse and fine focusing knobs are located at the base of the microscope on both sides. The user can place their hands on the table and take a relaxed pose while observing. The focusing adjustment is smooth and effortless. On the right, there is a coarse focusing lock knob for quick adjustments after changing the specimen. On the left, there is a coarse tension adjustment ring for further adjustment.

Stage
There is a fixed stage with a mechanical attachment for moving the laboratory ware in two mutually perpendicular directions. The smooth and subtle movement of the object augments the accuracy of the study – no part of the specimen will be overlooked. The travel knob is located at the bottom and, therefore, the user will not strain their right hand. The microscope set includes three dish holders for dishes of different sizes.

Reflected light illuminator
The fluorescence excitation light source: 5W LED. The illuminator has three excitation filters for the DAPI, FITC, and TRITC fluorochromes.
Unlike mercury lamps, LEDs do not require bulky power units; they use rather little electricity and do not contain harmful mercury vapors. Living cells under LED lighting are not damaged by UV radiation and remain viable much longer. LEDs will instantaneously reach full brightness, won’t heat up during prolonged use, and they turn off quickly when needed. All of that makes it easier to switch from the brightfield technique to fluorescence and back again. The lifetime of the LEDs is 50,000 hours. It is recommended to choose an LED-based fluorescence microscope for educational purposes as the safest and low-maintenance option.

Condenser
Phase contrast turret condenser with four positions. There is a free slot for brightfield microscopy; the other three slots with phase annuli plates are used for working with objectives at magnifications of 10x, 20x, and 40x. The phase contrast rings can be centered. Fast and intuitive switching between microscopy techniques saves time and simplifies the researcher’s work.

Transmitted light source
The transmitted light illuminator has a 5W LED. It is bright enough to work on all objectives using the brightfield and phase contrast techniques. The color temperature does not change when you adjust the brightness. The LED has a lifespan of 50,000 hours.

Köhler illumination in transmitted light
The Köhler illuminator improves the image quality of the observed sample: each objective achieves maximum resolution; the field of view is illuminated evenly without darkening at the edges. The object of study is in sharp focus and the image artifacts are removed.

Accessories
There is a line of accessories designed specifically for this microscope.
Eyepieces that extend the magnification range of the microscope. The optional eyepieces help you maximize the potential of the objective that you use most often.
A digital camera that outputs the microscope image to a monitor and stores files along with software that takes real-time measurements of specimens.
A calibration slide for measuring objects that can be combined with an eyepiece with a scale or the camera software.

Key features

Key features of the microscope:
  • Study of specimens in laboratory ware emitting fluorescence when exposed to excitation light
  • Microscopy techniques: fluorescence in reflected light, brightfield, and phase contrast in transmitted light
  • Trinocular head with the option to adjust eye relief
  • Two options for mounting a digital camera and monitor: a vertical tube of the trinocular head and a camera side port on the body; beam splitting on the trinocular head – 80:20, beam splitting on the body – 100:0/0:100
  • Transmitted and reflected light illuminators: energy-saving 5W LED with a lifetime of up to 50,000 hours
  • Köhler illumination
  • Tiltable stand for glassware up to 165mm
  • Fluorescence excitation filters for the DAPI, FITC, and TRITC fluorochromes
  • Phase-contrast turret condenser for quick and convenient changing of the microscopy technique
  • Wide range of compatible optional accessories

Key features of the camera:
  • For fluorescence and darkfield microscopy with 4x, 10x, 20x, or 40x objectives
  • 7.1MP resolution: When observing with low magnification objectives, the camera will enable you to see finer details
  • 51.3fps and 133.8fps at 3200x2200px and 1584x1100px resolutions for observing moving objects, recording video and moving the preparation without jerkiness and delays
  • Peltier element reduces the sensor temperature during prolonged operation and eliminates thermal noise and, therefore, long shutter speeds can be used when taking photos
  • Global shutter for fast signal readout from the sensor, increased image brightness, no false optical effects when observing moving objects
  • SONY Exmor monochrome CMOS backlit sensor provides low noise level and high light sensitivity even in low-light conditions. You will get clearer, brighter, and more color-saturated images
  • USB3.0 interface for fast, smooth data transfer
  • Software with photo, video recording, editing, external display functions, linear and angular measurements

Package

  • MAGUS CLM90 Digital Camera (digital camera, USB cable, 12V, 3A adapter, case, installation CD with drivers and software, user manual and warranty card)
  • Base with a built-in power input, reflected light illuminator, transmitted light source, condenser, focusing mechanism, stage, and revolving nosepiece
  • Trinocular head
  • Infinity plan achromatic objective: PLL 10x/0.25 phase WD 4.3 mm
  • Infinity plan achromatic objective: PLL 20х/0.40 WD 8.0mm
  • Infinity plan achromatic objective: PLL 40х/0.60 WD: 3.5mm
  • Infinity plan achromatic objective: PLL 10x/0.25 PHP2 WD 4.3mm
  • Infinity plan achromatic objective: PLL 20x/0.40 PHP2 WD 8.0mm
  • Infinity plan achromatic objective: PLL 40x/0,60 PHP2 WD 3.5mm
  • Eyepiece 10x/22mm with long eye relief (2 pcs.)
  • UV shield
  • C-mount adapter 1x
  • Hex key wrench
  • Power cord
  • Dust cover
  • User manual and warranty card

Available on request:
  • 10x/22mm eyepiece with a scale
  • 12.5x/14mm eyepiece (2 pcs.)
  • 15x/15mm eyepiece (2 pcs.)
  • 20x/12mm eyepiece (2 pcs.)
  • 25x/9mm eyepiece (2 pcs.)
  • Calibration slide

Microscope specifications

Microscope design: inverted

Image field: flat-field

Reflected light microscopy techniques: fluorescence

Transmitted light microscopy techniques: brightfield, phase contrast

Microscope magnification, x: 100–400 basic configuration (*optional: 100–500/600/800/1000)

Tube length: infinity (∞)

Microscope head type: trinocular

Microscope head: Siedentopf, rotatable 180°

Inclination: 45°

Microscope head magnification, x: 1

Interpupillary distance, mm: 48–75

Eyepiece diameter, mm: 30

Eyepieces, x/field, mm: 10х/22mm, eye relief: 10mm (*optional: 10x/22mm with a scale, 12.5x/14; 15x/15; 20x/12; 25x/9)

Revolving nosepiece: 6 objectives

Optical design: infinity plan achromatic objectives (∞), parfocal distance: 45mm

Objectives, x/aperture/working distance, mm: PLL 10x/0.25/4.3; PLL 20x/0.40/8.0; PLL 40x/0.60/3.5; phase: PLL 10x/0.25/4.3 PHP2; PLL 20x/0.40/8.0 PHP2; PLL 40x/0.60/3.5 PHP2

Spring-loaded retractable assembly: 40x

Stage: 227x208mm, fixed, with mechanical attachment and glass plates Ø118mm; Ø118mm; cup holders: 86x129.5mm, Ø87.5mm; 34x77.5mm, Ø68.5mm; 57x82mm, Ø60mm

Moving range, mm: 77/134.5

Condenser: NA 0.6, working distance: 55mm; phase contrast turret; with locking screws.

Diaphragm: adjustable iris diaphragm

Focusing mechanism: coaxial coarse and fine focusing knobs located on both sides

Fine focusing scale value, μm: 2

Coarse focusing tension adjusting knob: +

Coarse focusing lock knob: +

Illumination: transmitted and reflected light

Reflected light source: 5W LED

Transmitted light source: 5W LED, brightness-adjustable

Fluorescence filter: fluorochrome, excitation wavelength/ dichroic mirror/emission wavelength: DAPI, 365/35nm/400nm/450/65nm; FITC, 475/40nm/500nm/535/45nm; TRITC, 525/45nm/565nm/595/60nm

Phase contrast device: phase contrast condenser (turret) with a free slot and phase annuli plates for 10x, 20x and 40x objectives; centering telescope

Power supply, V/Hz: AC power supply, 85–265/50/60

Operating temperature range, °C (°F): 5… 35 (41… 95)

Operating humidity range, %: 20… 80

Camera specifications

Sensor: SONY Exmor CMOS

Color/monochrome: monochrome

Megapixels: 7.1

Maximum resolution, pix: 3200x2200

Sensor size: 1.1" (14.4x9.9mm)

Pixel size, μm: 4.5x4.5

Two-stage thermoelectric module (Peltier element) to set the temperature 42°C below room temperature: +

Light sensitivity: 3354mV with 1/30s

Signal/noise ratio: 0.15mV with 1/30s

Exposure: 0.1ms–1h

Video recording: +

Frame rate, fps at resolution: 51.3@3200x2200; 133.8@1584x1100; ADC digit capacity (bit); 8/12 (selectable)

Place of installation: trinocular tube

Image format: *.jpg, *.bmp, *.png, *.tif

Video format: recording: *.wmv, *.avi, *.h264 (Win 8 or newer), *h265 (Win 10 or newer)

Spectral range, nm: 380–650 (with IR filter and anti-reflective filter)

Shutter type: global shutter

White balance: auto/manual

Exposure control: auto/manual

Software features: image size, brightness, exposure time

Output: USB3.0, 5Gb/s

System requirements: Windows 8/10/11 (32 and 64 bit), Mac OS X, Linux, up to 2.8GHz Intel Core 2 or higher, minimum 2GB RAM, USB3.0 port, CD-ROM, 17" or larger display

Software: MAGUS View

Mount type: C-mount

Body: CNC aluminum alloy

Power supply: DC, 5V, from computer USB port; a 12V, 3A adapter for Peltier element

Operating temperature range, °C (°F): –10... 50 (14... 122)

Operating humidity range, %: 30… 80