MAGUS Epi-FL04 LED Epi-Fluorescence Attachment
85156
MAGUS Epi-FL04 LED Epi-Fluorescence Attachment, fluorescence filter cubes FITC/TRITC/DAPI
For fluorescence observations
The epi-fluorescence attachment is used to employ the fluorescence technique.
The fluorescence technique is based on the ability of substances to emit light when excited by the light of a certain wavelength. Some specimens exhibit autofluorescence, while others should be treated with fluorochromes.
For fluorescence observations
The epi-fluorescence attachment is used to employ the fluorescence technique.
The fluorescence technique is based on the ability of substances to emit light when excited by the light of a certain wavelength. Some specimens exhibit autofluorescence, while others should be treated with fluorochromes.
The attachment is mounted into the optical path between the microscope head and stand. There is a 4-position rotating turret inside the attachment with three narrow-band fluorescence filters for the fluorochromes DAPI, FITC, and TRITC. The required filter is introduced into the optical path by rotating the ring. One position of the turret corresponds to the brightfield observations.
The excitation light sources are LEDs. The LEDs instantly reach the brightness set by the user, do not heat up during prolonged use, and switch off quickly, if necessary. This makes it easy to switch from the brightfield technique to fluorescence and back again. The LED lifetime is 50,000 hours. For higher education, it is recommended to choose LED-based fluorescence as it is low-maintenance and the safest.
To enable the fluorescence technique, use the MAGUS Epi-FL04 LED attachment together with the MAGUS PlanF S-Apo fluorescent objective set. The plan semi-apochromatic fluorescent objective enhances the microscope’s resolving power and reduces chromatic and spherical aberrations. The image in the eyepieces and through the digital camera is cleaner, has more contrast, and it is brighter than with a regular plan achromatic objective.
The high numerical aperture of fluorescent objectives enhances the microscope’s resolving power, but at the same time, it reduces the depth of field. Therefore, for transmitted light observations, we recommend using regular objectives from the microscope set with a greater depth of field.
The excitation light sources are LEDs. The LEDs instantly reach the brightness set by the user, do not heat up during prolonged use, and switch off quickly, if necessary. This makes it easy to switch from the brightfield technique to fluorescence and back again. The LED lifetime is 50,000 hours. For higher education, it is recommended to choose LED-based fluorescence as it is low-maintenance and the safest.
To enable the fluorescence technique, use the MAGUS Epi-FL04 LED attachment together with the MAGUS PlanF S-Apo fluorescent objective set. The plan semi-apochromatic fluorescent objective enhances the microscope’s resolving power and reduces chromatic and spherical aberrations. The image in the eyepieces and through the digital camera is cleaner, has more contrast, and it is brighter than with a regular plan achromatic objective.
The high numerical aperture of fluorescent objectives enhances the microscope’s resolving power, but at the same time, it reduces the depth of field. Therefore, for transmitted light observations, we recommend using regular objectives from the microscope set with a greater depth of field.
- MAGUS Bio 270T Biological Microscope
- Epi-Fluorescence attachment
- FITC/TRITC/DAPI fluorescence filter cubes
- UV shield
Excitation light source: LEDs
Fluorescence filters: fluorochrome, excitation wavelength / dichroic mirror / emission wavelength: DAPI, 360–390nm / 415nm / 435–485nm; FITC, 460–495nm / 505nm / 510–550nm; TRITC, 528–553nm / 565nm / 578–633nm
Fluorescence filters: fluorochrome, excitation wavelength / dichroic mirror / emission wavelength: DAPI, 360–390nm / 415nm / 435–485nm; FITC, 460–495nm / 505nm / 510–550nm; TRITC, 528–553nm / 565nm / 578–633nm
