MAGUS Epi-FL05 LED Epi-Fluorescence Attachment
85158
MAGUS Epi-FL05 LED Epi-Fluorescence Attachment, fluorescence filter cubes B/G/UV/R
For fluorescence observations
The epi-fluorescence attachment is used to employ the fluorescence technique.
The fluorescence technique is based on the ability of substances to emit light when excited by the light of a certain wavelength. Some specimens exhibit autofluorescence, while others should be treated with fluorochromes.
For fluorescence observations
The epi-fluorescence attachment is used to employ the fluorescence technique.
The fluorescence technique is based on the ability of substances to emit light when excited by the light of a certain wavelength. Some specimens exhibit autofluorescence, while others should be treated with fluorochromes.
The attachment is mounted into the optical path between the microscope head and stand. There is a 5-position rotating turret inside the attachment with four fluorescence filters of different wavelengths: broad-band blue (В), green (G), ultraviolet (UV), and narrow-band red (R). The required filter is introduced into the optical path by rotating the ring. One position of the turret corresponds to the brightfield observations. The side knobs are used to adjust the opening of the field and aperture diaphragms.
The excitation light sources are LEDs. The LEDs instantly reach the brightness set by the user, do not heat up during prolonged use, and switch off quickly, if necessary. This facilitates the transition from the brightfield technique to fluorescence and back again. The LED lifetime is 50,000 hours. It is recommended to choose LED-based fluorescence for higher education as it is low-maintenance and the safest.
To employ the fluorescence technique, use the MAGUS Epi-FL05 LED attachment together with the MAGUS PlanF S-Apo fluorescent objective set. The plan semi-apochromatic fluorescent objective enhances the microscope’s resolving power and reduces chromatic and spherical aberrations. The image in the eyepieces and through the digital camera is cleaner, has more contrast, and it is brighter than with a regular plan achromatic objective.
The high numerical aperture of fluorescent objectives enhances the microscope’s resolving power, but at the same time, it reduces the depth of field. Therefore, for transmitted light observations, we recommend using regular objectives from the microscope set with a greater depth of field.
The excitation light sources are LEDs. The LEDs instantly reach the brightness set by the user, do not heat up during prolonged use, and switch off quickly, if necessary. This facilitates the transition from the brightfield technique to fluorescence and back again. The LED lifetime is 50,000 hours. It is recommended to choose LED-based fluorescence for higher education as it is low-maintenance and the safest.
To employ the fluorescence technique, use the MAGUS Epi-FL05 LED attachment together with the MAGUS PlanF S-Apo fluorescent objective set. The plan semi-apochromatic fluorescent objective enhances the microscope’s resolving power and reduces chromatic and spherical aberrations. The image in the eyepieces and through the digital camera is cleaner, has more contrast, and it is brighter than with a regular plan achromatic objective.
The high numerical aperture of fluorescent objectives enhances the microscope’s resolving power, but at the same time, it reduces the depth of field. Therefore, for transmitted light observations, we recommend using regular objectives from the microscope set with a greater depth of field.
- MAGUS Bio 290T Biological Microscope
- Epi-Fluorescence attachment
- B/G/UV/R fluorescence filter cubes
- UV shield
Excitation light source: LEDs
Fluorescence filters: filter type, excitation wavelength / dichroic mirror / emission wavelength: blue (B), 460–495nm / 505nm / 510nm; green (G), 510–550nm / 570nm / 575nm; ultraviolet (UV), 330–385nm / 410nm / 420nm; red (R), 620–650nm / 660nm / 670–750nm
Fluorescence filters: filter type, excitation wavelength / dichroic mirror / emission wavelength: blue (B), 460–495nm / 505nm / 510nm; green (G), 510–550nm / 570nm / 575nm; ultraviolet (UV), 330–385nm / 410nm / 420nm; red (R), 620–650nm / 660nm / 670–750nm
